Amyloid Fiber Formation in Human γD-Crystallin Induced by UV-B Photodamage

TitleAmyloid Fiber Formation in Human γD-Crystallin Induced by UV-B Photodamage
Publication TypeJournal Article
Year of Publication2013
AuthorsMoran SD, Zhang TO, Decatur SM, Zanni MT
Secondary TitleBiochemistry
Volume52
Issue36
Pagination6169–6181
Date Published08/2013
Abstract

γD-Crystallin is an abundant structural protein of the lens that is found in native and modified forms in cataractous aggregates. We establish that UV–B irradiation of γD-Crystallin leads to structurally specific modifications and precipitation via two mechanisms: amorphous aggregates and amyloid fibers. UV–B radiation causes cleavage of the backbone, in large measure near the interdomain interface, where side chain oxidations are also concentrated. 2D IR spectroscopy and expressed protein ligation localize fiber formation exclusively to the C-terminal domain of γD-Crystallin. The native β-sandwich domains are not retained upon precipitation by either mechanism. The similarities between the amyloid forming pathways when induced by either UV–B radiation or low pH suggest that the propensity for the C-terminal β-sandwich domain to form amyloid β-sheets determines the misfolding pathway independent of the mechanism of denaturation.

URLhttp://pubs.acs.org/doi/abs/10.1021/bi4008353
DOI10.1021/bi4008353
Short TitleBiochemistry